BEGIN:VCALENDAR
VERSION:2.0
PRODID:icalendar-ruby
CALSCALE:GREGORIAN
X-WR-CALNAME:High-Speed Atomic Force Microscopy (HS-AFM) Uncovers a Rare an
 d Transient Pentameric State in the Sensory Ion Channel TRPV3
X-WR-TIMEZONE:Eastern Time (US & Canada)
BEGIN:VEVENT
DTSTAMP:20260912T235425Z
UID:tag:localist.com\,2008:EventInstance_44827639781806
DTSTART:20231208T160000Z
DTEND:20231208T170000Z
DESCRIPTION:High-speed atomic force microscopy (HS-AFM) is a powerful techn
 ique that provides dynamic movies of biomolecules at work. First\, I will 
 briefly review our recent developments to break temporal limitations to ch
 aracterize molecular dynamics by developing HS-AFM line scanning (HS-AFM-L
 S) and HS-AFM height spectroscopy (HS-AFM-HS) [1]\, and resolution limitat
 ions by developing Localization AFM (LAFM) [2]. Then\, I will detail how w
 e used HS-AFM to analyze membrane-embedded Piezo1 [3] and TRPV3 [4] sensor
 y ion channels at the single-molecule level\, and discovered a previously 
 unobserved\, transient and reversible pentameric state in TRPV3 [4].\n\n \
 n\nReferences:\n\n[1] Heath et al.\, Nature Communications\, 2018\, 9(1):4
 983\, High-Speed AFM Height Spectroscopy (HS-AFM-HS): Microsecond dynamics
  of unlabeled biomolecules.\n\n[2] Heath et al.\, Nature\, 2021\, 594(7863
 ):385–390\, doi:10.1038/s41586-021-03551-x\, Localization Atomic Force M
 icroscopy.\n\n[3] Lin et al.\, Nature\, 2019\, 573(7773):230–234\, doi.1
 0.1038/s41586-019-1499-2\, Force-induced conformational changes in Piezo1.
 \n\n[4] Lansky et al.\, Nature\, 2023\, 621(7977)\, 206–214\, doi:10.103
 8/s41586-023-06470-1\, A pentameric TRPV3 channel with a dilated pore.
GEO:42.3783;-71.117081
LOCATION:Pierce Hall\, 209
SUMMARY:High-Speed Atomic Force Microscopy (HS-AFM) Uncovers a Rare and Tra
 nsient Pentameric State in the Sensory Ion Channel TRPV3
URL;VALUE=URI:https://events.seas.harvard.edu/event/high-speed_atomic_force
 _microscopy_hs-afm_uncovers_a_rare_and_transient_pentameric_state_in_the_s
 ensory_ion_channel_trpv3
CATEGORIES:Colloquia / Seminar / Lecture
END:VEVENT
END:VCALENDAR